StratiCELL (Les Isnes, Belgium) and Neuron Experts (Marseille, France) have developed a first-line compartmentalized model of coculture combining human sensory neurons and reconstructed epidermis (RHE-HSN).
This innovative model uses human sensory neurons derived from induced pluripotent stem cells (iPSC) growing in the immediate vicinity of 3D reconstructed human epidermis, i.e. on the backside of the polycarbonate support.
Up to now, the model allows to study the influence of ingredients or formulated products on processes involved in sensory skin and soothing properties, through the release of the neuropeptide CGRP upon capsaicin stimulation.
Epidermal morphology, cytokine release, gene expression and protein analysis are additional suitable biomarkers.