BARRIER FUNCTION

Skin is a barrier that protects us from external environmental aggressions, as well as from a mass loss of fundamental body water. StratiCELL offers multiple in vitro skin models and tools to address the efficacy of dermo-cosmetic ingredients on restoring the barrier function.

Skin is a chemical, physical and immune shield that protects us against aggressions from our environment (light, pollution, temperature, pathogens, etc.), and prevents loss of body water. The hydric barrier function is mainly ensured by the semi-permeable stratum corneum and hydrolipidic film, while intercellular tight junctions seal epidermal cells to form an efficient physical barrier. In the event of barrier disruption, immunomodulatory factors and cells are recruited to form a secondary front-line defence.

In order to address the efficacy of dermo-cosmetic ingredients to modulate those different barrier aspects, StratiCELL has developed specific and adapted in vitro skin models and assays to drive your innovation.

Efficacy on the epidermal barrier homeostasis can be assessed using in vitro 2D human keratinocytes monolayer cells or 3D reconstructed epidermis, and the recording of multiple key skin barrier biomarkers.

 Available endpoints :

– Trans-epidermal dye diffusion

Expression of up to 96 genes selected for their role in the barrier function and the epidermal biology (by individual RT-qPCR or 96 TaqMan Low Density Array technology)

– Expression of 48 genes selected for their role in the biological processing of epidermal lipids (by TaqMan Low Density Array technology)

Immunostaining and quantification of barrier-related biomarkers (e.g. FLG, LOR, IVL, TGM-1, ZO-1, Claudin, Desmosome).

Long-chain ceramides are among the main lipid component of the inter-corneocyte cement. Discover the activity of your ingredient on improving the biological level of ceramides using the ceramide-impaired 3D skin model designed by StratiCELL.

Available endpoints :

-Expression of individual targeted genes involved in ceramide synthesis and elongation (by TaqMan RT-qPCR)

-Expression of 48 genes selected for their role in the biological processing of epidermal lipids (by TaqMan Low Density Array technology)

Disruption of the skin barrier integrity is often associated with an inflammatory response, as observed in atopic dermatitis (AD). StratiCELL has developed an AD-like reconstructed epidermis model with typical features of Th2-induced inflammatory disorder to study the efficacy of dermo-cosmetic ingredients to restore barrier and reduce inflammation.

Available endpoints :

Expression of 96 genes involved in inflammation and pruritis (by TaqMan Low Density Array technology)

Expression of 48 genes involved in the biological processing of epidermal lipids (by TaqMan Low Density Array technology)

Trans-epidermal dye diffusions (i.e. biotin release or lucifer-yellow penetration).

Immunostaining and quantification of AD and barrier function biomarkers (e.g. CA2, FLG, LOR, IVL, ABCA12)

Morphological analysis (Hemalun&Eosin staining)

Exposure to air pollution deeply impacts the skin barrier, increasing its susceptibility to Reactive Oxygen Species (ROS) and other oxidative stress. Anti-oxidative efficacy of ingredients can be measured using StratiCELL’s in vitro skin models mimicking oxidative stress conditions.

Available endpoints :

Expression of 96 genes involved in inflammation and oxidative-stress response to pollution

-Quantification of Reactive Oxygen Species (ROS)

-Quantification of pro-inflammatory factors (e.g. IL-1a, IL-6, IL-8)