BARRIER FUNCTION

Skin is the body’s first line protection against physical and oxidative deterioration by the environmental exposome. Skin is also the essential hydric barrier preventing a mass loss of fundamental water.

This double barrier function is mainly ensured by the upper layer of the epidermis, producing a semi-permeable stratum corneum and hydrolipidic film. 

Long-chain ceramides are among the main lipid constituent of the hydrolipidic film. Ingredients able to improve the natural ceramide content could help restoring the hydration and, to a larger extend, the barrier function of disordered skins.

Discover the activity of your ingredient on improving the biological level of ceramides using the “ceramide-impaired 3D skin model” designed by StratiCELL.

Available endpoints :

-Expression of gene involved in the biological processing of long-chain ceramides

-Trans-epidermal dye diffusions : biotin release and lucifer-yellow penetration

-Immunostaining and quantification (e.g. ZO-1, Claudin, Desmosome)

Skin barrier disruption is associated with a Th2 type pro-inflammatory response. StratiCELL has developed a 3D skin model highly relevant to study effect of dermo-cosmetic ingredients on restoring barrier resilience and normal inflammation level.

Available endpoints :

-Expression of up to 96 genes involved in inflammation

-Immunostaining and quantification (e.g. CA2, FLG, LOR, IVL)

-Spongiosis and morphological analysis by Hemalun&Eosin staining

Barrier-damaged skins are more susceptible to Reactive Oxygen Species (ROS) and oxidative stress. Anti-oxidative efficacy of ingredients can be measured using in vitro skin models mimicking in vivo oxidative stress conditions.

Available endpoints :

-Expression of up to 96 genes involved in anti-oxidation

-Quantification of Reactive Oxygen Species