StratiCELL is renowned for its integrated gene expression profiling services, which range from single gene analysis by reverse-transcription quantitative PCR (RT-qPCR) to complete transcriptome profiling via DNA microarrays. Services also include the selection of the best in vitro models and assays, biological material collection, RNA extraction, quality assessment, quantification of gene expression levels and data analysis with a comprehensive biological interpretation focusing on cell and tissue functions directly related to the specifics of skin biology.
Analysing gene expression changes using RT-qPCR TaqMan Low Density Arrays (TLDA) offers the advantage of focusing on up to 96 target genes, carefully selected and identified as key factors in dermo-cosmetic benefits. StratiCELL has developed several standardized arrays to meet the objectives of different studies. However, the preparation of a customized array is always possible.
StratiCELL relies on the unique expertise of its highly skilled and passionate scientists to properly interpret the large-scale expression data with regards to dermo-cosmetics benefits.
|Resarch Topic||Cell and tissue functions||Model compatibility|
|Epidermal barrier & homeostasis||Epidermal biology, cell junctions, barrier, antioxidant, antimicrobial, lipid synthesis & transport, melanogenesis.||NHEK, RHE, RHE-MEL|
|Melanogenesis||Melanin synthesis, melanogenic factors & signal transduction, melanosome biogenesis, maturation & transport, melanosome transfer, apoptosis.||NHEM, RHE-MEL|
|Dermal biology, re-modeling & ageing||Extracellular matrix components & enzymes, cell proliferation, antioxidant, proteasome, glycosaminoglycans synthesis & elongation, dermo-epidermal junction.||NHDF|
|Wound healing||Extracellular matrix components & enzymes, cell adhesion, inflammatory cytokines & chemokines, growth factors, TGF signaling.||NHEK, NHDF|
|Inflammation||Chemokines & receptors, interleukins & receptors, innate immune response, inflammatory response.||NHEK, RHE, NHDF|
|Glycosaminoglycans||Glycosaminoglycans synthesis & elongation, transferases, metabolism & degradation.||NHDF|
|Sensitive skin & barrier resilience||Barrier function, cell junctions, lipid synthesis & transport, pruritus, inflammation, immune response.||NHEK, RHE|
|Pollution & detoxification||Biotransformation & metabolism enzymes of phases I and II, antioxidant & oxidative stress response, inflammatory response, barrier function & terminal differentiation, autophagy, unfolded protein response.||NHEK, NHDF, RHE, RHE-MEL|
|Epidermal lipids & corneocyte lipid envelope||Ceramide and long-chain ceramide metabolism, cholesterol and fatty acid metabolism, regulation of lipid homeostasis, corneocyte-bound lipid envelope.||RHE|
|Customized array||Tailored to your needs.||Contact us to assess feasibility.|
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