Taqman arrays

StratiCELL is renowned for its integrated gene expression profiling services, which range from single gene analysis by qRT-PCR to complete transcriptome profiling via DNA microarrays. Services also include the selection of the best in vitro models and assays, biological material collection, RNA extraction, quality assessment, quantification of gene expression levels and data analysis with a comprehensive biological interpretation focusing on cell and tissue functions directly related to the specifics of skin biology.

Taqman arrays

Analysing gene expression changes using qRT-PCR TaqMan low density arrays offers the advantage of focusing on 96 to 384 target genes, carefully selected and identified as key factors in dermo-cosmetic benefits. StratiCELL has developed several standardized arrays to meet the objectives of different studies. However, the preparation of a customized array is always possible.

Research topic	Cell and tissue functions	Culture model's compatibility
Epidermal barrier & homeostasis	Epidermal biology, cell junctions, barrier, antioxidant, antimicrobial, lipid synthesis & transport, melanogenesis.	NHEK, RHE, RHE-MEL
Melanogenesis	Melanin synthesis, melanogenic factors & signal transduction, melanosome biogenesis, maturation & transport, melanosome transfer, apoptosis, …	NHEM, RHE-MEL
Dermal biology, re-modeling & ageing	Extracellular matrix components & enzymes, cell proliferation, antioxidant, proteasome, glycosaminoglycans synthesis & elongation, dermo-epidermal junction, …	NHDF
Wound healing	Extracellular matrix components & enzymes, cell adhesion, inflammatory cytokines & chemokines, growth factors, TGF signaling, ...	NHEK, NHDF
Inflammation	Chemokines & receptors, interleukins & receptors, innate immune response, inflammatory response.	NHEK, RHE, NHDF
Glycosaminoglycans	Glycosaminoglycans synthesis & elongation, transferases, metabolism & degradation.	NHDF
Sensitive skin & barrier resilience	Barrier function, cell junctions, lipid synthesis & transport, pruritus, inflammation, immune response.	NHEK, RHE
Pollution & detoxification	Biotransformation & metabolism enzymes of phases I and II, antioxidant & oxidative stress response, inflammatory response, barrier function & terminal differentiation, autophagy, unfolded protein response, …	NHEK, NHDF, RHE, RHE-MEL
Customization	Tailored to your needs.	Contact us to assess feasibility

NHEK : Normal Human Epidermal Keratinocytes in monolayer cultures

NHEM : Normal Human Epidermal Melanocytes in monolayer cultures

NHDF : Normal Human Dermal Fibroblasts in monolayer cultures

RHE : Human Reconstituted Epidermis (3D epidermal tissue culture at the air-liquid interface)

RHE-MEL : Human Reconstituted Epidermis with Human Melanocytes (NHEM)

StratiCELL relies on the unique expertise of its highly skilled and passionate scientists to properly interpret the large-scale expression data with regards to dermo-cosmetics benefits.