The skin microbiota describes the natural flora that lives on our skin. Its impact and influence of our skin balance have been largely described. This is why new skincare products are now evolving to better preserve or enhance this natural ecosystem.
In the MicroBIOS Platform, StratiCELL combines skin microbiota components and 3D reconstructed epidermis, to study the efficacy of dermo-cosmetic ingredients on the equilibrium of the microflora.
The microbial flora and its diversity, also called microbiota, is beneficial and even essential to maintaining our good health. StratiCELL is studying commensal and opportunistic pathogens strains of the skin including Staphylococcus epidermidis and S. aureus, Cutibacterium acnes, and probiotics such as Lactobacillus plantarum.
ENDPOINTS:
-Individual- or co-infections in ratios, on top of reconstructed human epidermis (RHE)
-Bacterial adhesion on top of RHE, by colony units counting (CFUs)
-Bacterial growth on top of RHE, by colony units counting (CFUs)
-RHE tissue morphology : Hemalun&Eosin staining, GRAM staining
-RHE response to colonization : immunolabelling and expression of key epidermal genes
Microbiote-Friendly skin care products preserve the natural flora of the skin, including the commensal Staphylococcus epidermidis. Confirm the safeness of your product by measuring its impact on the growth of S. epidermidis in the real context of an in vitro 3D reconstructed epidermis.
Acne is an inflammatory skin disease associated with an increase of Cutibacterium acnes phylotype IA1. C. acnes stimulates sebaceous gland, but also local keratinocytes, leading to inflammation characterized by an increase of IL-1, TNF-α or the chemokine IL-8. As a consequence, the production of β-defensin 2 is enhanced too.
StratiCELL evaluates the influence of ingredients on the adhesion and growth of Staphylococcus aureus in the real context of a 3D reconstructed epidermis. Inflammatory response of the tissue is evaluated at the gene and the protein levels. Anti-inflammatory efficay testing using 3D Atopic Dermatitis model is also available at StratiCELL.
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